The CyanoTag Project 

CyanoTag uses the power of high-throughput approaches and fluorescent tagging to discover more about the proteins of a model photosynthetic bacteria. Our pipeline for scarless endogenous fluorescent protein tagging in the model cyanobacterium Synechococcus elongatus PCC7942 allows us to determine subcellular localisation, track relative protein abundances and to elucidate protein-protein interaction networks. As of early 2024 we have targeted over a quarter of the 2714 proteins in S. elongatus. Associated imaging, expression and interactome data is publicly available and is shared via an online multi-omics database (MORF) and our recent preprint/publication.  Detailed methods and additional resources will also be available to download from this page. 
So far the CyanoTag project has provided insights into processes as diverse as photosynthesis and bacterial cell division, relevant to cyanobacteria and more broadly to all photosynthetic life.  Our tagged lines, datasets and methods are intended to be shared, so as to facilitate future discoveries in these fascinating organisms. 

Current libraries and datasets

CyanoTag Project Highlights

Imaging data for over 500 tagged lines...
Protein expression data for over 500 proteins...

​The first preliminary interactome map for a cyanobacterium, based on AP-MS data and an initial 82 bait proteins...

For more information and biological insights, check out our preprint and data repository

Documents & Datasets

Methods document version 1.3 (June 2024)
Contains detailed, step-by-step methods for generating and working with tagged cyanobacteria.  Updated to include pictures.
cyanotagmethods_v1p4_ajperin_19jun2024.pdf
File Size: 5378 kb
File Type: pdf
Download File
Making your own CyanoTag plasmids or lines?  
Download our primer and eblock designs for the approaches detailed in the Methods document. 

Backbone plasmids pLM433 and pLM434 are deposited in Addgene.
cyanotag_cloningprimerdesigns_3apr2024.xlsx
File Size: 1849 kb
File Type: xlsx
Download File
cyanotag_bsai_eblockdesigns_3apr2024.xlsx
File Size: 1001 kb
File Type: xlsx
Download File
Browse our data for over 700 protein targets in MORF via ​https://morf-db.org/projects/York-Mackinder-Lab/MORF000032 or via our latest data table.
cyanotagalldata_june2024_ajp.xlsx
File Size: 161 kb
File Type: xlsx
Download File